FastQCFastQC Report
Sat 3 Nov 2018
HVCHFBGX7_n01_ba141.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHFBGX7_n01_ba141.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36550366
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC533190.14587815618590522TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC390020.1067075497958078TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC145300.058.65432444
CGTCTGA252300.058.37381416
GTCGTAT81500.058.06156542
TCGTATG125950.057.9674343
ATGCCGT134000.056.65265347
ACGTCTG262400.056.08724615
ACACGTC262700.056.06354513
TGTCGTA85850.055.16044641
GATGTCG103850.054.73306739
TATGCCG156200.054.35917746
TGCCGTC138450.052.85992448
GATCGCG16500.052.81844739
GATCGGA283250.052.4374661
TCGCGTA10950.052.42035341
ATCGGAA284550.052.3875662
CACGTCT283300.051.99912614
GCCGTCT125550.051.15489649
CCGTCTT134800.050.7863350
TCTCGTA62900.050.3023241
ATGTCGT104700.050.1769540
Produced by FastQC (version 0.11.5)