FastQCFastQC Report
Sat 3 Nov 2018
HVCHFBGX7_n01_ba140.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHFBGX7_n01_ba140.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41342438
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC2386790.5773220244050435TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT290350.062.03200542
TCGTATG301100.062.025843
TCTCGTA289700.061.77264841
CGTATGC302300.061.70981644
ATGCCGT302250.060.70071847
AATCTCG295950.060.5394339
TGCCGTC305500.059.56233648
GCCGTCT299450.059.2581849
TATGCCG315200.058.85085346
CGTCTGA335150.058.71064416
CCGTCTT303850.058.67652550
ATCTCGT305550.058.56834440
CGTCTTC305550.056.95238551
ACGTCTG347000.056.7860315
ACACGTC349600.056.53417213
GATCGGA372650.053.7316931
ATCGGAA375850.053.537512
CACGTCT373800.052.88329714
TCGGAAG385650.052.140863
GTATGCC362150.051.6950945
Produced by FastQC (version 0.11.5)