FastQCFastQC Report
Sat 3 Nov 2018
HVCHFBGX7_n01_ba139.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCHFBGX7_n01_ba139.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44826497
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1505680.33589062290546595TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA44550.064.8937541
CGATGTA399800.064.5115934
CACCGAT405650.063.780231
ACCGATG405600.063.66739732
CCGATGT408300.063.16880433
TATCGCG80500.062.91361639
GTCACCG414500.062.4687529
CGCGTAT45800.062.28181542
CGTCTGA420450.062.240916
ATCGCGT64400.061.79390340
CTCGTAT207750.061.34070642
CGTATGC215250.061.28463444
TCTCGTA209950.061.19825741
TCGTATG195150.061.03241743
ACGTCTG432150.060.71790715
ACACGTC432250.060.68004213
GTATCGC86100.060.4882238
TCACCGA429200.060.41068630
TATCTCG254600.060.0339339
ATGCCGT201600.059.65258847
Produced by FastQC (version 0.11.5)