FastQCFastQC Report
Mon 23 Oct 2017
HVCCMAFXX_n03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCCMAFXX_n03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88201370
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGGGCAT1055700811.96921090908225No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGC55547996.297860225980617No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGCATCCGCCGTGGGCGGT39852764.518383331233971No Hit
GAAAACCTTGTTACGACTTTTGCCCGGTTCAAACAACAGCGATTGGAAGG38319584.344556099298685No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGA23392832.6522071029055443No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGT21991642.4933444911343217No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCACAAACCACACCGTGGTAATC16492701.8698915901192918No Hit
GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGACCAA12837061.4554263726289058No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCACAGACCACACCGTGGTAATC9724031.1024806077275218No Hit
GGCTACCTTGTTACGACTTCACCCCAATCACCAGTTTTACCTTCGGCGGC8160740.9252395966185106No Hit
GGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAACGCG8065470.9144381770940745No Hit
GGCTACCTTGTTACGACTTCACCCTAATCATCTGTCCTACCTTAGACGGC7619610.8638879418766399No Hit
GGAAACCTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATAAAGGCG7101660.8051643642269954No Hit
GGCTACCTTGTTACGACTTCACCCCAATCGCTGACCCTACCTTCGGCCGC6296940.7139276861572558No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCTGTCCCACCTTAGACGGC5387670.6108374507107996No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCTGCCCTGCCTTAGACGGC4669910.5294600299292404No Hit
AGTAACCTTGTTACGACTTGTACTTTCTCTAAATGTCCAAACTTGCACAA4530800.5136881660681688No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCTGTCCCGCCTTAGGCGGC4136490.46898251126938284No Hit
GGATACCGTGTTACGACTTCTTCATCCTTTAAGACATGCAACTAAATCAA3893530.44143645387821073No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGGTAAGATTTACACAA3777690.42830287103250214No Hit
AGGTACCTTGTTACGACTTTTGCCCGGTTCAAGCCATTGCGATTAAACTG3630330.4115956475505993No Hit
GAAAACCTTGTTACGACTTTTGCCCGGTTCAAACAACAGCGATTGAAAGG3425290.38834884310753903No Hit
AGATACCTTGTTACGACTTAACCTTCCTTGCTAAATCTCAGTTCGAACAC2923900.3315027873149816No Hit
GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGACCAG2845480.32261176895551624No Hit
AGATACCTTGTTACGACTTAACCTTCCTTGCTAAATCTCAGTTCGACAAC2773900.3144962487544128No Hit
AGATACCTTGTTACGACTTTTACCCGGTTCAAGCCATTGCGATTGAACTG2767940.31382052228893953No Hit
GGAAACCGTGTTACGACTTTTGCATCCTTTAGAGGGCTCTATTTACGCAA2676600.30346467407479044No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC2547230.2887971014509185No Hit
GGTTACCTTGTTACGACTTAACCCCAGTCGCTGCACCCACCGTAGACGGT2516150.28527334666116866No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2512910.2849060054282604No Hit
GGCTACCTTGTTACGACTTCACCCTAATCATCTGTCCCACCTTAGACGGC2471980.2802654879396998No Hit
GAAAACCTTGTTACGACTTCTTCTTTTTCTAAGGGTTGAGGTTTACAAAA2360500.26762622848148504No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCTGTCCCACCTTAGGCGGC2315210.2624913876054306No Hit
AGATACCTTGTTACGACTTAACCTTCCTTGCTAAATCTCAGTTTGAACAC2207650.2502965656882654No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAGTGGA2112240.23947927339450625No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGATTCCACTGTGGGGGAT1633650.18521821146315526No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGACCAA1604030.1818599869820616No Hit
AGATACCTTGTTACGACTTAACCCTCCTTGCTAAATCTCAGTTCGACAAC1391920.1578116076881799No Hit
GGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAA1355660.15370056043347172No Hit
GGCTACCTTGTTACGACTTCACCCCAATCGCTGACCCTACCTTAGGCCGC1335410.15140467772779492No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCGGCCCTACCGTAGACGGC1316460.1492561850229764No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACTAG1257640.14258735436875866No Hit
AGATACCGTGTTACGACTTGACCATTCTCTAGATAATTGGGTTTACAAGA1193560.13532216109568368No Hit
GGATACCGTGTTACGACTTCTTCATCCTCTAGATGATAAGGCTCACGGCA1091840.12378946041314325No Hit
GGCTACCTTGTTACGACTTCACCCCAATCGCTGACCCTACCTTAGGTCGC1003990.11382929766283675No Hit
AGATACCATGTTACGACTTGACCATTCTCTAGATAATTGGGTTTACAAGA978030.11088603272262099No Hit
GGCTACCTTGTTACGACTTCACCCTCCTTAAAAAACCTAGATTCGAATAT928240.10524099568974948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG51804700.093.923989
CTTGTTA50690200.093.9179157
CCTTGTT50745650.093.910376
ACCTTGT50724200.093.882455
ACCGTGT1073200.093.612085
AAACCTT27017100.093.6018753
TTGTTAC50842350.093.469618
TCTCGAT4016150.093.41608494
GATACCT1998550.093.211722
GGCTACC6459050.093.094141
CTCATGG2840200.093.0477594
GTTACCT14534750.092.854612
CGTGTTA1085000.092.7760247
TACCTTG23488100.092.699584
AACCTTG27902850.092.53914
CCTGAGG1858450.092.37717494
GTAACCT519700.092.322912
GGTACCT435250.092.1940462
GAAACCT22658050.092.114082
AGGTACC418700.092.096631
Produced by FastQC (version 0.11.2)