FastQCFastQC Report
Mon 23 Oct 2017
HVCCMAFXX_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCCMAFXX_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88201370
Sequences flagged as poor quality0
Sequence length12
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGG3465037239.28552583707033No Hit
GGTGTCTATTGT11915831.3509801491745537No Hit
ATCCCGAATTTG9040481.0249818115070095No Hit
GAAGTTGGAAGT8079520.9160311228725811No Hit
TGCCTACAAGCA7142650.8098116843309803No Hit
CTAACCTCCGCT6549440.74255535940088No Hit
TCTAGCGTAGTG6404070.7260737559972141No Hit
CCTTATAGGCGA6189600.7017578071633127No Hit
ACGGGACATGCT6125070.694441594274556No Hit
TACACGATCTAC5872940.6658558704927146No Hit
GACAGGAGATAG5764960.6536134302675798No Hit
GAGGCTCATCAT5692050.6453471187579058No Hit
CTAGCGAACATC5673570.6432519132072438No Hit
GGTAATCCTGTG5662120.6419537474304537No Hit
CTATTTGCGACA5659980.6417111208136563No Hit
AAGGAGCGCCTT5611590.6362248114740168No Hit
CAAGCATGCCTA5462040.6192692925291297No Hit
CACGCCATAATG5382820.6102875726306746No Hit
AAGAGATGTCGA5267450.5972072769391223No Hit
GCGGCAATTACG5240760.5941812468445785No Hit
CAGTAACGGCCA5170250.5861870399518737No Hit
TCCGAATTCACA5151970.5841145097859591No Hit
GATAGTGCCACT5151760.5840907006319742No Hit
TCACAGATCCGA5100370.5782642605211235No Hit
GACGGAACCCAT5077430.5756633938905938No Hit
TTCAACAGGTGC5032030.5705160815529283No Hit
ACACGTAAGCCT5004160.5673562666883746No Hit
GTCTAATTCCGA4830600.547678567804559No Hit
AATGCCTCAACT4764860.5402251688380804No Hit
ACCGTGCATAGG4733140.5366288528171388No Hit
GACTTGGTATTC4703750.5332967050285046No Hit
TAGGAACTGGCC4691930.5319565897899319No Hit
AGTAGAGGGATG4689610.5316935553268617No Hit
TGCTCGTAGGAT4610660.5227424471978157No Hit
CGACTCCAAGAT4569910.5181223375555277No Hit
TAATGACCACGC4427300.5019536544613763No Hit
ATAAGGCCTTCT4412550.5002813448362536No Hit
ATGACTCATTCG4317680.4895252760813125No Hit
CTACTGATATCG4186230.47462187945606743No Hit
CTCAGTATGCAG4145110.46995982035199685No Hit
CTTCGGCAGAAT4141520.4695527971957806No Hit
ACTCTTCTAGAG4102800.465162842708679No Hit
ATCGAATGCGGC4044450.4585472992086177No Hit
GACAATCTGCTT4042310.4583046725918203No Hit
CCACGAAGCTAT4007870.4543999713383136No Hit
TGAGCCGGAATC3982030.45147031162894635No Hit
TCTATCGGGTTA3897760.4419160382656188No Hit
CAACCTTTACAG3827760.43397965360402No Hit
AAGACCGGTGCT3817520.4328186739049518No Hit
GTTGACGACAGC3789240.42961237450166595No Hit
TCGAGACGCTTA3745430.42464533147274247No Hit
ATGTGCACGACT3731940.4231158767715286No Hit
ATTCCTGTGAGT3700620.41956491152008185No Hit
CGATGTCGTCAA3654300.4143132924125782No Hit
ACGCGCAGATAC3629800.41153555778101863No Hit
GTATGCGCTGTA3595830.4076841436816685No Hit
CAGGGTGATACC3520170.39910604563171753No Hit
GCCTCCCTTATG3510710.3980334999331643No Hit
AATCAGTCTCGT3474950.39397914114032473No Hit
GAGTGGTAGAGA3466160.39298255798067533No Hit
ACAAAGGCCCTG3462810.392602745286156No Hit
GTCAATTGACCG3444290.39050300465854443No Hit
GCACGACAACAC3442450.3902943911188681No Hit
CGGAGCTATGGT3385710.38386138446602364No Hit
TAGGATTGCTCG3360530.3810065535263228No Hit
CTTGTGTCGATA3355760.3804657456000967No Hit
GGAAACCACCAC3300580.3742096069482821No Hit
ATGCACTGGCGA3296170.3737096147146014No Hit
GCACCCAAAGTG3203930.3632517272690889No Hit
GCTGATGAGCTG3183080.36088781840916984No Hit
CTAGAGACTCTT3116240.35330970482658036No Hit
CGTGTTTCAAAC3110530.3526623225920414No Hit
CTTATCAAGGCG3093730.3507575902732577No Hit
GGATCGCAGATC3069520.34801273494958185No Hit
NNNNNNNNNNNN3027950.34329965622982955No Hit
GGCCACGTAGTA2991910.3392135518983435No Hit
TCCAAAGTGTTC2947540.3341830177921273No Hit
ATCGATCTGTGG2937900.3330900642473014No Hit
AATCCGTACAGC2898920.32867063176002825No Hit
TCCGACACAATT2879760.3264983299012249No Hit
TCCTCTGTCGAC2850560.32318772372810084No Hit
GCACACACGTTA2842390.3222614342611685No Hit
CATAGTTTAGCC2792840.31664360768999394No Hit
ACCTAGAGTCGG2787370.3160234359171519No Hit
ATTCTAGCGTTG2775070.3146288997551852No Hit
GACTTTCCCTCG2757030.3125835800509675No Hit
GGAGTCGGTCTA2756840.31256203843545743No Hit
ATGGCAGCTCTA2748740.3116436853531867No Hit
TTCAGACTACCA2694030.30544083385552856No Hit
ACGCATTGCATT2679910.30383995169236033No Hit
TATCGTTGACCA2660930.3016880576798297No Hit
CATCAAACCTCA2653920.30089328544443245No Hit
CAGGCGTATTGG2640230.2993411553584712No Hit
CCAGTGTATGCA2638140.2991041975878606No Hit
CCTCGTTCGACT2625330.297651839194788No Hit
TTGCCACTAGAT2610740.29599766987746334No Hit
CCTGAACTAGTT2586970.2933027004002319No Hit
AACGGATAAACG2567630.29110999069515586No Hit
TGAACTGCCGTA2477450.28088565971254187No Hit
TACAGATGGCTC2433760.27593222191446687No Hit
TTCCTAGGTGAG2326540.26377594815137223No Hit
TTGAAGTCCATC2319810.2630129214546214No Hit
ACGTGTACCCAA2288940.25951297581885635No Hit
TTACTGTGCGAT2269420.2572998582675077No Hit
GGTTTCAAGCTT2267860.2571229902664777No Hit
CTTGCCTTGTGC2200600.24949725837591866No Hit
ATGAGACTCCAC2182260.24741792559457976No Hit
ACCTGTCTCTCT2169030.24591794889353757No Hit
GAATCTTCGAGC2155050.2443329394996926No Hit
AGCTGTTGTTTG2153700.24417988065264748No Hit
TGGCTCTACAGA2095010.23752578899851554No Hit
AGATTCGTTGTC2092110.2371969959196779No Hit
ACACCTGGTGAT2028550.22999075864694618No Hit
TCCCATTGAAGG2005190.22734227370844692No Hit
GCGCTAAAGTTC1957910.22198181275415566No Hit
CCTTTCTTCTCA1940650.22002492705045285No Hit
TTGCGCATACTA1933260.21918707158403547No Hit
TCGAGGACTGCA1925430.2182993302711738No Hit
CCTACGCCTCTA1894320.21477217417371183No Hit
GCGGATGTGACT1866230.21158741638593595No Hit
AATTTGATCCCG1788560.2027814307192734No Hit
GGCTCTGCTCTT1766670.20029960985866774No Hit
GAACTAGTCACC1758980.19942774131512922No Hit
GCAATAGCTGCT1745800.19793343346027392No Hit
TCTCACTAGGTA1722470.19528834982948676No Hit
ACGTTAGCACAC1687610.19133603026801058No Hit
CCACTAATTGAC1665580.1888383366380817No Hit
GGAGACAAGGGA1645890.1866059450096977No Hit
CATTCGATGACT1593860.18070694366765505No Hit
CTGCATAGATCT1577280.1788271542720935No Hit
ATTTCGACATGC1548610.1755766378685501No Hit
TGCTCAGTATGT1533800.17389752562800329No Hit
TGAGAACCTCCA1514340.17169121069207882No Hit
AGAGTCCTGAGC1510310.17123430168941822No Hit
AGGAGTAACACA1436670.16288522502541627No Hit
GAGTCAACCGCA1428000.1619022470966154No Hit
GGATGGTGTTGC1425770.1616494165566816No Hit
AGGCTGATTTGC1408820.15972767769933732No Hit
CCTGTATCCCGT1388920.15747147691696853No Hit
ACCACCAGAACG1369520.1552719645964683No Hit
GAGTGAGTACAA1329180.15069833949291264No Hit
AATGGCCTCTAT1233050.13979941581406277No Hit
AGGCTACACGAC1177700.13352400308521284No Hit
AGTTCAGACGCT1101070.12483592941923691No Hit
CGATCCGTATTA1042880.11823852622697356No Hit
ACGCCACGAATG1024500.1161546583686852No Hit
AGCAGTCGCGAT950610.10777723747374898No Hit
TGCCCATTTAAG929600.10539518830603198No Hit
CGCAGCGGTATA892290.10116509528139983No Hit
TGCCAGGCTAGA889370.10083403466408741No Hit
GATCTATCCGAG884310.10026034742997755No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGATT704.695915E-46.0002391
TCGGGAC704.695915E-46.0002391
CATGTGT704.695915E-46.0002391
TCCGATT751.7508064E-46.00023841
GGTTTCT550.0091580846.00023841
ACGTGTC600.00339555796.00023841
ACCAGTC1156.825212E-86.00023841
GTCCGGC4000.06.00023841
ATAAGGG550.0091580846.00023841
GGAGTCT1259.673386E-96.00023841
CATCGTT600.00339555796.00023841
CGACTGA600.00339555796.00023841
ATCGATT550.0091580846.00023841
TGCCAGG90900.06.0002381
ACGTGAC650.00126158496.0002381
CTTGATG48450.06.0002381
TTTGCAG550.0091601296.0001096
CCGCTTA1850.06.00010876
CGCTGGA650.00126191246.00010876
GCACCTT1601.0913936E-116.00010876
Produced by FastQC (version 0.11.2)