FastQCFastQC Report
Mon 23 Oct 2017
HVCCMAFXX_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVCCMAFXX_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88201370
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACTACCGATTGAATGATTTAGTGAGGTCTTCGGACCGGGGGGCGAGG1034833311.732621613473803No Hit
ACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAG54468496.175469836806389No Hit
GCTGCCCGGAACTGAGCAATATCGAGAGGCAGGAAGAGATATAAATAATT38777514.396474793985626No Hit
ACACCATGGGAGTTGTATTCGCCTTAAGTCGGGATACTAAATTGGTTACC30923093.5059648166462716No Hit
GCTACTACCGATTGGATGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGG21262652.410693847499194No Hit
GCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGGG20440522.317483277187191No Hit
ACACCATGGGAGCGGATTGCACCAGAAGTAGTTAGCTTAACCGAAAGGAG9202291.0433273315369136No Hit
GCTGCCCGGGACTGAGCCGTTTCGAGAAAAGCGGGGACTGCTGTTTCTCA8081360.9162397364122575No Hit
ACACCATGAGAGTCTGTAACACCCAAAGCCGGTGGGATAACCTTTATAGG7098100.8047607423784914No Hit
ACACCATGGGAGTTGTATTCGCCTTAAGTCGGAATGCCAAACTGGCTACC7013860.7952098703228759No Hit
GCTGCCCGGGACTGAGCCGTTTCGAGAAAAGCGGGGACTGCTGTTTCGAA6866880.7785457300719932No Hit
ACACCATGGGAGCGAATTGCACCAGAAGTAGTTAGCTTAACCCGCAAGGG6068050.6879768420830651No Hit
ACACCATGAGAGCCGGGGGGACCCGAAGTCGGTAGTCTAACCGCAAGGAG5598740.6347679180039948No Hit
ACACCATGAGAGTTGGCAATACCCGAAGTCCGTGAGCTAACCGCAAGGAG5581390.6328008283771556No Hit
ACACCATGAGAGTTTGTAACACCCAAAGCCGGTGGGGTAACCTTTTGGAG5222570.5921189206018002No Hit
GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATGCGCTTAGCCTG5070080.5748300735011259No Hit
ACACCATGGGAGCGAATTGCACCAGAAGTAGTTAGCTTAACCTGCAAAGG4681940.530823954321798No Hit
GTTACTATCGATGAATGTTCAATTGAGTTCTTAGGACGGCCACTACGGTT4591230.5205395335696033No Hit
ACACCATGGGAGCGAATTGCACCAGAAGTAGTTAGCTTAACCTGAAAGGG4429330.5021838096165626No Hit
ACACCATGGGAGTTTGTAACGCCCAAAGTCGGTGGCCTAACCTTTATGGA4036200.45761193958778645No Hit
GCTTCAACTGATGAGATCGTACAACAATAACAAATCTGTAAGTCAGGTTT3933210.4459352502120999No Hit
GCACCTACCGATTGAATGGTCCGATGAACACTTTGGATTTTGATATATTG3695860.4190252373630931No Hit
GCTGTCCGGGACTGAGCTGTTTCGAGAAGACTGAGGACCGCTTTTGTGAG3647750.41357067356210003No Hit
GCTGCCCGGAACTGAGCAATATCGAGAGGCAGGAAGAGATACAAAAAATA3449020.391039277507821No Hit
GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCAGCTTAGAGAA3186090.3612290829496186No Hit
ACACCATGGAAGTCTGCAATGCCCGAAGCCGGTGGCCTAACCACTTATGT3083790.34963062365131065No Hit
AAACCATCCGAGTTGGGTTTCAGTGAGGTCACCTCTAATTAGGGTGTTCG2844630.3225153985703397No Hit
GCTGTCCGGGACTGAGCTGTCTCGAGAGGACTGCGGACTGCTGTATCGAG2711100.30737617794372124No Hit
AAACCATGGGAGTTGTATTCGCCTTAAGCCGGGATGCTAAATTGGCTACC2488740.282165685181534No Hit
GCTTCTATTGATTGAGTGTTGTGGTGAATTATTCGGATGAATTCGATAGT2407620.2729685491279784No Hit
ACACCATGAGAGCCGGGGGGACCCGAAGTCGGTAGTCTAACCGTAAGGAG2403190.2724662893558229No Hit
ACACCATGAGAGTTTGTAACACCCAAAGTCGGTGGGGTAACCTTTTGGAG2229460.25276931639497213No Hit
GCTGCAACCGATCGGAGGGTCCTGTGAATTCATCGGATTGACCATCTTCG2160180.24491456311846405No Hit
AAACCATCCGAGTTGGGTTTCAGTGAGGTTGCCTCTGATTAGGGTGTTCA2153730.24418328196035957No Hit
AAACCATCCGAGTTGGGTTCCAGTGAGGCTGCCTCTAATTAGGGTTGTCG1898820.21528237033052888No Hit
GGTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCAGCTTAGAGAA1742260.19753207915024448No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAG1640880.1860379266217747No Hit
ACACCATGGGAGTTGATTTCACTCGAAGTGGAAATACTAAACTAGTTATC1585600.1797704502775864No Hit
GCTACTACCGATTGAATGGCTCAGTGAGGCCTTGGGATTGGCTTAGGAGG1568860.1778725205742269No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1506610.17081480707159086No Hit
GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCTGCTTAGAGAA1470480.16671849881696849No Hit
GCATCAATCGATTGGATAATTCGGTGAATGATTCGGATTGAGTTGTTATT1451140.16452578911189247No Hit
GGTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATGCGCTTAGCCTG1360260.1542220942826625No Hit
ACACCATGAGAGTTTGTAACACCCAAAGCCGGTGGGGTAACCTTTTAGGA1357940.15395905981959238No Hit
GCACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAG1260800.142945625447768No Hit
AAACCACCCGAGTTGGGTTTCAGTGAGGCTGCCTCTTTTTGGGGTTGTCG1253320.1420975660582143No Hit
GCTTCAACCGATTGGATGATCCGGCAAACTATACGGACATTTGAAATCAC1113310.12622366296577933No Hit
GCTACTACCGATTGGATGGCTTAGTGAGGCCCTCGGATCGGCCCCGCCGG1017840.11539956805659594No Hit
GCTCCTACCGATTGAATGGTCCGGTGAAGTGTTCGGATCGCGGCGACGTG992260.11249938634740027No Hit
ACACCACCCAAAAAGGGCTTGGATGAGGATGTAATGTTTTTTGTTATATT887120.10057893658567889No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCCTCGGGAG885720.10042020889244692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTAC14698150.093.868232
TACTACC14802350.093.867263
ACTACCG14815250.093.837454
CTAGTAC1367750.093.831772
CCGATTG20732950.093.831138
ACCATGG15903600.093.8226243
CGGAACT4557150.093.82267
CGATTGA17934800.093.8021859
CTCCTAC3268000.093.7992862
GCCCGGA4561850.093.797284
CATGGGA15650300.093.794395
TGGGAGT12608750.093.760177
CCATGGG15642400.093.756814
AGTACCG2046800.093.746994
CCGGAAC4574850.093.740096
ATGGGAG15696300.093.7297746
CCATGAG4339300.093.695924
CCGGGAC2408100.093.675776
CATGAGA4336350.093.6654745
ATGAGAG4348200.093.6553046
Produced by FastQC (version 0.11.2)