FastQCFastQC Report
Tue 29 Sep 2020
HVC5JBGXG_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC5JBGXG_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36921090
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC16273004.407508012358248No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT15833264.288405353146399No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC14648673.9675616294101825No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG14371783.892566552070917No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA13405303.6307974656219524No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT11649353.1552020809786496No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA6956571.8841724336957548No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCACTAGGTCCACTATG662090.17932569163044754No Hit
AACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATGA400420.10845291945606156No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG1722800.0143.50141
ATCGATT1456800.0143.209731
TCGATTC1477900.0141.902852
CGATTCC1485000.0141.199423
GATTCCT1502200.0139.69274
ACTGTAT1322550.0139.157363
GATAACG1655450.0139.005485
ATTCCTT1511750.0138.887885
AACAATG1718000.0138.713752
CGATAGG1808800.0137.717156
CTGTATC1336900.0137.586874
TCCTTGG1534850.0137.185757
CCTTGGT1535950.0137.076578
CGATACA1852600.0137.06635
AACGCAT1688500.0137.028388
ACGCATT1688600.0136.9779
TAACGCA1693600.0136.651447
ATACAGG1857500.0136.537117
ACAATGG1742950.0136.305343
GATACAG1863150.0136.251276