FastQCFastQC Report
Tue 29 Sep 2020
HVC5JBGXG_n01_0920_CD8_prestim.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC5JBGXG_n01_0920_CD8_prestim.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12383669
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC1158557493.55526217633884No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCC289620.23387253002321043No Hit
GATCGATAACGCATTTCTTGTCGAAAGGACGAAACACCGCCAGTAGGTCC269660.21775452816124202No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCA254630.20561757585736504No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCC245140.19795425733681996No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCAGTAGGTCCA167520.1352749334627726No Hit
GATCGATAACGCATTTCTTGTGGAAAGAACGAAACACCGCCAGTAGGTCC144300.11652443229869919No Hit
GATCGATAACGCATTTCTTGTGGAAAAGACGAAACACCGCCAGTAGGTCC142650.11519203234517977No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTCC142350.11494977780817624No Hit
GATCGATAACGCATTTCTTATGGAAAGGACGAAACACCGCCAGTAGGTCC124690.10068906072990161No Hit
GATCGATAACGCATTTCTTGTAGAAAGGACGAAACACCGCCAGTAGGTCC124010.10013995044602694No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAC12309950.0144.882644
ATCGATA12318550.0144.877472
GATCGAT12298950.0144.867191
ATAACGC12310600.0144.859096
GATAACG12303300.0144.822425
TCGATAA12325550.0144.75933
AACGCAT12320000.0144.726998
ACGCATT12321350.0144.679939
TAACGCA12325100.0144.678857
CGTCTGA11973400.0134.37955145
GTCTGAA910900.0119.418335145
CGTCTAA50250.091.6162145
CTCTGAA37850.087.3441145
CGTCGAA35650.086.02318145
CGTTGAA33950.077.517815145
TCTGAAC148650.076.81574145
CGCTGAA25600.069.384224145
GATACGC12050.066.196765
ACACATT23050.065.109279
ATAACTC23450.064.320826
Produced by FastQC (version 0.11.5)