FastQCFastQC Report
Tue 29 Sep 2020
HVC5JBGXG_n01_0920_CD8_presort.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC5JBGXG_n01_0920_CD8_presort.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19470975
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG1741042589.41732501839276No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCACTAGGTCCACTATG7941034.078393608948704No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCCACTATG531910.2731809783536777No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCACTATG363790.18683707415781695No Hit
TAACAATGGTCTTTTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG326160.16751087195171274No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCACTATGA271830.13960780084202254No Hit
TAACAATGGTCTTGTGGAAAGCACGAAACACCGCCAGTAGGTCCACTATG259020.1330287774495114No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCAGTAGGTCCACTATGA256370.13166777729415194No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGAAGGTCCACTATG242090.1243337840041395No Hit
TAACAATGGTCTTGTGGAAACGACGAAACACCGCCAGTAGGTCCACTATG217770.11184339767268973No Hit
TAACAATGGTCTTGTGGAAAGAACGAAACACCGCCAGTAGGTCCACTATG200580.10301487213660333No Hit
TAACAATGGTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTCCACTATG198780.10209041920088748No Hit
TAACAATGGTCTTGTGGAAAAGACGAAACACCGCCAGTAGGTCCACTATG197960.10166927953017246No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTC19344100.0144.878945
AACAATG19380500.0144.833222
ACAATGG19349950.0144.82843
TAACAAT19385000.0144.807071
CAATGGT19354550.0144.805594
GGTCTTG19279750.0144.789648
ATGGTCT19362150.0144.771216
TGGTCTT19366150.0144.69967
GTCTTGT19327300.0144.44739
AACTCCA18867200.0132.9753145
ACTCCAG1591250.0121.71175145
GGTCTTT91000.082.9375848
AACTCAG125050.079.0213145
CAATGAT42500.078.487854
CAATAGT29650.073.616534
CTCCAGT286050.072.38495145
AATGATC49650.067.039435
AACCCAG68250.065.01009145
GATCTTG50850.064.4450458
ACAATGA54250.061.488183
Produced by FastQC (version 0.11.5)