FastQCFastQC Report
Tue 29 Sep 2020
HVC5JBGXG_n01_0920_CD8_CFSE_low.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC5JBGXG_n01_0920_CD8_CFSE_low.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19017053
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG1697804389.27799170565493No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCACTAGGTCCACTATG7758454.079733069051236No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCCACTATG579960.30496838810934584No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCACTATG328640.17281331655330615No Hit
TAACAATGGTCTTTTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG315220.16575649234400305No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCACTATGA258440.13589907963131828No Hit
TAACAATGGTCTTGTGGAAAGCACGAAACACCGCCAGTAGGTCCACTATG247730.13026729220347652No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGAAGGTCCACTATG237180.1247196397885624No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCAGTAGGTCCACTATGA236630.12443042568162374No Hit
TAACAATGGTCTTGTGGAAAAGACGAAACACCGCCAGTAGGTCCACTATG207230.10897061705617585No Hit
TAACAATGGTCTTGTGGAAACGACGAAACACCGCCAGTAGGTCCACTATG206410.10853942511492186No Hit
TAACAATGGTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTCCACTATG204790.10768755810902982No Hit
TAACAATGGTCTTGTGGAAAGAACGAAACACCGCCAGTAGGTCCACTATG200760.10556840747091571No Hit
TAACAATGGTCTTGTAGAAAGGACGAAACACCGCCAGTAGGTCCACTATG193640.10182439939563717No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAATGG18886300.0144.88733
CAATGGT18890900.0144.88124
AATGGTC18893850.0144.880845
AACAATG18922350.0144.853492
TAACAAT18932450.0144.827931
GGTCTTG18829200.0144.789768
ATGGTCT18911500.0144.77136
TGGTCTT18917000.0144.676547
GTCTTGT18884450.0144.406469
CTGAACT18225400.0129.23036145
CTGAACA182750.0122.70357145
CGTATGC98250.0113.7116145
TGAACTC2010950.0104.44362145
CGAACTC48500.097.61265145
CTAACTC53400.092.04985145
CTGAACC56350.083.4998145
CAATGAT38750.080.0957644
GGTCTTT93700.077.685558
CTGACTC37800.074.60922145
CAATAGT32150.066.313784
Produced by FastQC (version 0.11.5)