FastQCFastQC Report
Tue 29 Sep 2020
HVC5JBGXG_n01_0920_CD8_CFSE_high.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC5JBGXG_n01_0920_CD8_CFSE_high.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17183047
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT1612346393.83355001007679No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCCACTAT398860.23212413956616657No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCACTAT312430.18182456231423916No Hit
ATACTGTATCTCTTGTGGAAAAGACGAAACACCGCCAGTAGGTCCACTAT262260.15262717956832683No Hit
ATACTGTATCTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTCCACTAT254960.1483788061570221No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCACTATG252870.14716249102967593No Hit
ATACTGTATCTCTTGTGGAAAGAACGAAACACCGCCAGTAGGTCCACTAT252690.14705773661679444No Hit
ATACTGTATCTCTTGTGGAAAGCACGAAACACCGCCAGTAGGTCCACTAT251540.14638847231227384No Hit
ATACTGTATCTCTTGTGGAAACGACGAAACACCGCCAGTAGGTCCACTAT226150.13161228040637962No Hit
ATACTGTATCTCTTGTAGAAAGGACGAAACACCGCCAGTAGGTCCACTAT225800.13140859127022117No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCAGTAGGTCCACTATG210240.12235315424557705No Hit
ATACTGTATCTCTTGTGAAAAGGACGAAACACCGCCAGTAGGTCCACTAT209900.12215528479902313No Hit
ATACTGTATCTCTTATGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT202370.11777305852681424No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCACTAGGTCCACTAT201990.11755191032184222No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTGT17088550.0144.963941
CTGTATC17098600.0144.895284
ACTGTAT17096850.0144.874483
GTATCTC17113050.0144.79036
TACTGTA17108100.0144.786852
TGTATCT17116450.0144.772985
ATCTCTT17126150.0144.737358
TATCTCT17139200.0144.72027
TCTCTTG17089650.0144.658319
GTCTGAA16546950.0131.44383145
TCTGAAC1559500.0114.812706145
AGGAATG50250.094.50099145
GACTGAA49300.087.64567145
GTCGAAC54850.080.49554145
GCTGAAC40750.074.36691145
GTCTAAC68100.073.1376145
AGGAATC55250.071.777145145
ATATCTC34500.066.635446
CTATATC37200.061.7984624
ACTATAT37550.061.2220843
Produced by FastQC (version 0.11.5)