FastQCFastQC Report
Tue 29 Sep 2020
HVC5JBGXG_n01_0920_CD8_CD_low.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC5JBGXG_n01_0920_CD8_CD_low.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17184647
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC1602832293.27117397290733No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCC472220.2747917952577088No Hit
GATCGATAACGCATTTCTTGTCGAAAGGACGAAACACCGCCAGTAGGTCC378120.22003361488891798No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCA344110.20024269337624453No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCC312100.1816156014144486No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCAGTAGGTCCA227670.13248453692415096No Hit
GATCGATAACGCATTTCTTGTGGAAAAGACGAAACACCGCCAGTAGGTCC223380.1299881225375185No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTCC216220.12582161274537673No Hit
GATCGATAACGCATTTCTTGTGGAAAGAACGAAACACCGCCAGTAGGTCC204870.11921688004414638No Hit
GATCGATAACGCATTTCTTGTAGAAAGGACGAAACACCGCCAGTAGGTCC191000.11114572210881027No Hit
GATCGATAACGCATTTCTTATGGAAAGGACGAAACACCGCCAGTAGGTCC189780.11043578608277493No Hit
GATCGATAACGCATTTCTTGTGAAAAGGACGAAACACCGCCAGTAGGTCC177220.10312693650326363No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACGCCAGTAGGTCCA177220.10312693650326363No Hit
TATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC173720.10109023478922785No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAC17070250.0144.927344
ATAACGC17081050.0144.871346
GATAACG17066100.0144.827445
ATCGATA17097200.0144.763382
TCGATAA17097750.0144.745993
AACGCAT17096700.0144.737678
TAACGCA17103450.0144.694527
ACGCATT17099950.0144.682629
GAGCACA16630800.0130.438145
AGCACAC1688050.0107.435104145
GAGCACC69650.0101.0716145
TTACCTA77850.083.5343145
GATCGAT33855200.072.975161
TCACCCT186100.071.83656145
GATACGC17650.068.613995
CTATAAC33100.068.3537754
ACACATT30900.067.105229
GAGCCAC30450.065.47513145
ATAACTC30750.065.088796
AATAACG37000.065.069475
Produced by FastQC (version 0.11.5)