FastQCFastQC Report
Tue 29 Sep 2020
HVC5JBGXG_n01_0920_CD8_CD_high.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC5JBGXG_n01_0920_CD8_CD_high.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23417452
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC2187022193.3928294162832No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTC623670.2663270111539035No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCACCAGTAGGTC455420.1944788869429518No Hit
CGATCGATACAGGTATTCTTGTCGAAAGGACGAAACACCGCCAGTAGGTC380170.16234473332111451No Hit
CGATCGATACAGGTATTCTTGTGGAAAAGACGAAACACCGCCAGTAGGTC351270.15000351020256175No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTC326290.13933625229593724No Hit
CGATCGATACAGGTATTCTTCTGGAAAGGACGAAACACCGCCAGTAGGTC323060.1379569391238637No Hit
CGATCGATACAGGTATTCTTGTGGAAAGAACGAAACACCGCCAGTAGGTC318950.13620183784298992No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCAGTAGGTCC302490.1291728920806585No Hit
CGATCGATACAGGTATTCTTATGGAAAGGACGAAACACCGCCAGTAGGTC299530.12790887753287591No Hit
CGATCGATACAGGTATTCTTGTAGAAAGGACGAAACACCGCCAGTAGGTC289080.12344639374087325No Hit
CGATCGATACAGGTATTCTTGTGAAAAGGACGAAACACCGCCAGTAGGTC276190.11794195201083363No Hit
CGATCGATACAGATATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC271750.11604593018915979No Hit
CAATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC255920.10928601455017395No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACGCCAGTAGGTCC235160.10042083143802323No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAT23242350.0144.906832
CGATCGA23233200.0144.872121
CGATACA23283100.0144.870575
TCGATAC23287050.0144.87034
ATCGATA23285550.0144.863133
ATACAGG23255650.0144.852867
TACAGGT23258200.0144.811138
ACAGGTA23255400.0144.776819
GATACAG23296900.0144.728736
CGTCTGA22595450.0133.4436145
GTCTGAA1887850.0113.36109145
CGACTGA62800.080.23337145
CGTCTGT68050.079.15721145
CGTCTAA82150.078.98512145
ATACAGA55500.076.422717
CGTCGAA63400.076.38658145
CTCTGAA69950.073.483246145
TACAGAT57850.072.815518
CGTTGAA68100.071.434044145
AATCGAT58450.069.725412
Produced by FastQC (version 0.11.5)