FastQCFastQC Report
Tue 29 Sep 2020
HVC5JBGXG_n01_0920_CD4_prestim.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC5JBGXG_n01_0920_CD4_prestim.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17582360
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA1647212293.68550069501478No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCCA465950.2650099304075221No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACAGCCAGTAGGTCCA336230.19123143878296203No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCA316500.18000996453263385No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCAC264970.15070218104964295No Hit
ATCGATTCCTTGGTTCTTGTCGAAAGGACGAAACACCGCCAGTAGGTCCA248000.14105046194026286No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCAGTAGGTCCAC233540.13282631000616527No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCCCCAGTAGGTCCA203270.11561019112337594No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGATT17488500.0144.897251
CGATTCC17480050.0144.882253
TCGATTC17492200.0144.879472
ATTCCTT17499500.0144.860845
GATTCCT17492000.0144.833834
TTCCTTG17493200.0144.796946
TCCTTGG17487350.0144.720387
CCTTGGT17486450.0144.716648
CTTGGTT17487800.0144.618019
TCTGAAC16927900.0134.2985145
CTGAACT1253000.0121.691696145
TCTGAAA165500.0116.91817145
TCAGAAC47050.079.66403145
TGAACTC202950.075.62462145
CCTTAGT24250.072.650868
TCTAACT54250.067.22018145
CTATTCC36900.067.014593
TCTGAAT50250.065.50149145
ACTGAAC30950.064.885925145
TCGAACT46200.064.18193145