FastQCFastQC Report
Tue 29 Sep 2020
HVC5JBGXG_n01_0920_CD4_presort.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC5JBGXG_n01_0920_CD4_presort.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25462633
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT2363072792.8055122971768No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCTCCAGTAGGT807370.3170803270816494No Hit
ACGATCTATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT727500.2857127933313102No Hit
ACGATCGATAGGTAAGGTCTTCTGGAAAGGACGAAACACCGCCAGTAGGT546390.21458503525538775No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCACCAGTAGGT474050.1861747761906634No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAAGACGAAACACCGCCAGTAGGT431210.1693501218039784No Hit
ACGATCGATAGGTAAGATCTTGTGGAAAGGACGAAACACCGCCAGTAGGT415020.1629917848637256No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGAACGAAACACCGCCAGTAGGT399120.15674734030844337No Hit
ACGATCGATAGGTAAGGTCTTATGGAAAGGACGAAACACCGCCAGTAGGT396920.155883329112115No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACAAAACACCGCCAGTAGGT393160.15440665543111745No Hit
ACGATCGATAGGTAAGGTCTTGTAGAAAGGACGAAACACCGCCAGTAGGT371600.1459393457070995No Hit
ACGATCGATAGGTAAGGTCTTGTGAAAAGGACGAAACACCGCCAGTAGGT360440.1415564525475429No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCAGTAGGTC336570.1321819310673802No Hit
ACGATCGATAGATAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT327110.12846668292316824No Hit
ACGATCGATAGGTAAAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT268050.10527190962537142No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACGCCAGTAGGTC264130.10373239876645907No Hit
ACAATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT255150.1002056621559915No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG25189650.0144.931351
GATCGAT25214600.0144.888033
CGATCGA25205050.0144.886262
ATCGATA25243650.0144.884174
CGATAGG25177600.0144.876256
ATAGGTA25300100.0144.812298
GATAGGT25194600.0144.806557
TCGATAG25240850.0144.758015
TAGGTAA25315300.0144.634849
ACGTCTG24583750.0134.61586145
CGTCTGA1772600.0122.286095145
CTATAGG116150.0111.013096
ACGATCT122450.0108.674771
CGATCTA122850.0108.320492
GATCTAT125200.0105.129923
ATCTATA128500.0102.373844
ACGACTG61800.0102.29613145
TATAGGT135300.095.116267
TCTATAG150350.087.014625
ACGTCTA87300.083.460884145
Produced by FastQC (version 0.11.5)