FastQCFastQC Report
Tue 29 Sep 2020
HVC5JBGXG_n01_0920_CD4_CFSE_low_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC5JBGXG_n01_0920_CD4_CFSE_low_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18269383
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT1701915293.15668733859266No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCCACTAT506800.27740400428410744No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCACTAT430690.2357441408940849No Hit
ATACTGTATCTCTTGTGGAAAAGACGAAACACCGCCAGTAGGTCCACTAT313110.1713850982269078No Hit
ATACTGTATCTCTTGTGGAAAGCACGAAACACCGCCAGTAGGTCCACTAT309210.16925037917262997No Hit
ATACTGTATCTCTTGTGGAAAGAACGAAACACCGCCAGTAGGTCCACTAT289040.15821005011499295No Hit
ATACTGTATCTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTCCACTAT286970.15707700692464543No Hit
ATACTGTATCTCTTGTGGAAACGACGAAACACCGCCAGTAGGTCCACTAT268560.14700003826073382No Hit
ATACTGTATCTCTTGTAGAAAGGACGAAACACCGCCAGTAGGTCCACTAT265130.14512258022068944No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCACTATG264570.14481605645904955No Hit
ATACTGTATCTCTTATGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT237650.1300810213459316No Hit
ATACTGTATCTCTTGTGAAAAGGACGAAACACCGCCAGTAGGTCCACTAT236680.1295500784016625No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCAGTAGGTCCACTATG224380.12281750292278616No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCACTAGGTCCACTAT219900.120365312829667No Hit
ATACTATATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT195900.10722858018795708No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTGT18158500.0144.942631
CTGTATC18168400.0144.842864
ACTGTAT18166300.0144.842063
TACTGTA18176700.0144.787122
ATCTCTT18187000.0144.772748
GTATCTC18175550.0144.770326
TGTATCT18180800.0144.775
TATCTCT18202900.0144.741857
TCTCTTG18142700.0144.700249
TGAACTC17586950.0133.71616145
GAACTCC1405350.0114.79234145
TGAACTA91200.084.66095145
TGAACCC50650.084.30711145
TGAACAC58300.081.32753145
TAACTCC59050.076.61134145
ACTATAT41100.074.636583
ATATCTC41850.073.300016
CTATATC43200.071.008614
GATCTCG89300.065.84121145
AGAACTC44950.062.095394145
Produced by FastQC (version 0.11.5)