FastQCFastQC Report
Tue 29 Sep 2020
HVC5JBGXG_n01_0920_CD4_CFSE_low_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC5JBGXG_n01_0920_CD4_CFSE_low_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17064784
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC1591792393.27936995862356No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTC467080.27370988112126116No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCACCAGTAGGTC338940.19861956647092635No Hit
CGATCGATACAGGTATTCTTGTCGAAAGGACGAAACACCGCCAGTAGGTC326780.19149378040765122No Hit
CGATCGATACAGGTATTCTTCTGGAAAGGACGAAACACCGCCAGTAGGTC278340.1631078365832231No Hit
CGATCGATACAGGTATTCTTGTGGAAAAGACGAAACACCGCCAGTAGGTC260480.15264183830278777No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTC243200.1425157212655021No Hit
CGATCGATACAGGTATTCTTGTGGAAAGAACGAAACACCGCCAGTAGGTC235710.13812656521172492No Hit
CGATCGATACAGGTATTCTTATGGAAAGGACGAAACACCGCCAGTAGGTC224020.1312762001558297No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCAGTAGGTCC218150.12783636757429806No Hit
CGATCGATACAGGTATTCTTGTAGAAAGGACGAAACACCGCCAGTAGGTC216870.1270862848307954No Hit
CGATCGATACAGGTATTCTTGTGAAAAGGACGAAACACCGCCAGTAGGTC207890.12182398558340966No Hit
CGATCGATACAGATATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC200200.1173176291009602No Hit
CAATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC182810.10712705182790477No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACGCCAGTAGGTCC171240.10034700702921291No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAC16970600.0144.882524
ATCGATA16971950.0144.871863
CGATCGA16935300.0144.86981
CGATACA16968800.0144.853445
ATACAGG16945000.0144.828197
ACAGGTA16935050.0144.801979
TACAGGT16947300.0144.773048
GATACAG16971750.0144.731266
GATCGAT16967450.0144.711122
CACGTCT16425750.0132.78885145
CACGTCG70400.0108.95421145
GATCTCG61850.0102.56504145
ACGTCTG1385800.094.32486145
CCGCGCG259850.093.77278145
CAAGTCT47400.091.00591145
CACGTTG33950.090.97055145
ATACAGA35250.086.385387
CACGCTG28100.084.108955145
CACTCTG43900.079.60008145
GCTCGAT23850.072.971052
Produced by FastQC (version 0.11.5)