FastQCFastQC Report
Tue 29 Sep 2020
HVC5JBGXG_n01_0920_CD4_CFSE_high_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC5JBGXG_n01_0920_CD4_CFSE_high_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA11673.88535031847134No Hit
GATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA138.280254777070063No Hit
GATAGGTCGCTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA31.910828025477707No Hit
GATAGGTCGCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA21.2738853503184715No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACAT10.6369426751592357No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCATAGGTCCACTAT10.6369426751592357No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGATCCACTA10.6369426751592357No Hit
GATAGGTCGCAACTTGAGGAAAGGACGAAACACCGCCAGTAGGTCCACTA10.6369426751592357No Hit
GATAGGTCGCCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA10.6369426751592357No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACTGCCAGTAGGTCCACTA10.6369426751592357No Hit
GATAGGTCGCATCTTGTGGAAGGACGAAACACCGCCAGTAGGTCCACTAT10.6369426751592357No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGACCACTA10.6369426751592357No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGGAGGTCCACTA10.6369426751592357No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACATAGTTGAAAAAGTGGCAC10.6369426751592357No Hit
GATAGGTCGCATCTTGTCGAAAGGACGAAACACCGCCAGTAGGTCCACTA10.6369426751592357No Hit
GATAGGTCGCATCTTGTGGATAGGACGAAACACCGCCAGTAGGTCCACTA10.6369426751592357No Hit
GATAGGTCGCATCTTATGGAAAGGACGAAACACCGCCAGTAGGTCCACTA10.6369426751592357No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGCCCACTA10.6369426751592357No Hit
GATAGGTCACATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA10.6369426751592357No Hit
GATAGGTCGCATCTTGTGAAAAGGACGAAACACCGCCAGTAGGTCCACTA10.6369426751592357No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTGCACTA10.6369426751592357No Hit
GATAGGTAAGGTCTTTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT10.6369426751592357No Hit
GATAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATGAGT10.6369426751592357No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTT10.6369426751592357No Hit
GATAGGTCGCATCTTGTCCACTATGAGTAGCACCTGTGTCATACTACCGC10.6369426751592357No Hit
AATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA10.6369426751592357No Hit
GATAGGTAATGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA10.6369426751592357No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAT150.0145.06
ATAGGTC150.0145.02
TCGCATC150.0145.07
AGGTCGC150.0145.04
GCATCTT150.0145.09
CGCATCT150.0145.08
GATAGGT150.0145.01
GGTCGCA150.0145.05
TAGGTCG150.0145.03
TCTGAAC150.005374226396.666664145
Produced by FastQC (version 0.11.5)