FastQCFastQC Report
Tue 29 Sep 2020
HVC5JBGXG_n01_0920_CD4_CFSE_high_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC5JBGXG_n01_0920_CD4_CFSE_high_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10631421
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT984799392.63101329540049No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCTCCAGTAGGT359440.3380921515571625No Hit
ACGATCTATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT315410.29667717984265696No Hit
ACGATCGATAGGTAAGGTCTTCTGGAAAGGACGAAACACCGCCAGTAGGT234220.22030921360371297No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCACCAGTAGGT193420.18193240583737583No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAAGACGAAACACCGCCAGTAGGT191650.1802675296181009No Hit
ACGATCGATAGGTAAGATCTTGTGGAAAGGACGAAACACCGCCAGTAGGT180420.1697045014020233No Hit
ACGATCGATAGGTAAGGTCTTATGGAAAGGACGAAACACCGCCAGTAGGT173380.16308262084626315No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACAAAACACCGCCAGTAGGT171110.16094744061024394No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGAACGAAACACCGCCAGTAGGT168110.15812561650977794No Hit
ACGATCGATAGGTAAGGTCTTGTAGAAAGGACGAAACACCGCCAGTAGGT163420.15371416483271616No Hit
ACGATCGATAGGTAAGGTCTTGTGAAAAGGACGAAACACCGCCAGTAGGT158830.14939677395900322No Hit
ACGATCGATAGATAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT143280.13477031903825462No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCAGTAGGTC136910.12877864586493187No Hit
ACGATCGATAGGTAAAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT118390.11135858508472198No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACGCCAGTAGGTC111190.10458620724360367No Hit
ACAATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT111090.1044921464402548No Hit
ATGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT106820.10047575013725824No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG10511050.0144.915631
CGATAGG10503600.0144.884436
ATCGATA10538600.0144.843674
GATCGAT10526550.0144.833133
GATAGGT10509400.0144.820057
ATAGGTA10561050.0144.768818
TCGATAG10535500.0144.73215
TAGGTAA10565300.0144.613839
CGATCGA10551000.0144.403352
CACACGT10226650.0132.30344145
ACGATCT52550.0114.535441
CTATAGG53300.0110.068736
CGATCTA54700.0110.0335852
ACACGTC893850.0107.11221145
GATCTAT56500.0105.245123
AACACGT32500.099.49102145
CACACGG24750.097.83711145
ATCTATA62000.095.909314
TATAGGT61950.094.680387
ACAATCG18850.091.55881
Produced by FastQC (version 0.11.5)