FastQCFastQC Report
Tue 29 Sep 2020
HVC5JBGXG_n01_0920_CD4_CD_low.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC5JBGXG_n01_0920_CD4_CD_low.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23355546
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA2160050892.4855621016096No Hit
GATAGGTCGCATCTTGAGGAAAGGACGAAACACCGCCAGTAGGTCCACTA913480.39111909436842107No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCCACTA620290.2655857413909313No Hit
GATAGGTCGCATCTTGTGGAAACGACGAAACACCGCCAGTAGGTCCACTA486220.2081818168584027No Hit
GATAGGTCGCATCTTGTGGAAAAGACGAAACACCGCCAGTAGGTCCACTA473110.20256858906231523No Hit
GATAGGTCGCATCTTGTGGAAAGGACAAAACACCGCCAGTAGGTCCACTA442890.18962947815478173No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCACTA438290.18765992454211947No Hit
GATAGGTCGCATCTTGTGGAAAGAACGAAACACCGCCAGTAGGTCCACTA421450.18044964566446017No Hit
GATAGGTCGCATCTTATGGAAAGGACGAAACACCGCCAGTAGGTCCACTA405580.1736546857007753No Hit
GATAGGTCGCATCTTGTAGAAAGGACGAAACACCGCCAGTAGGTCCACTA377630.16168750668470777No Hit
GATAGGTCGCATCTTGTGAAAAGGACGAAACACCGCCAGTAGGTCCACTA371350.15899863783959495No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCACTAT334160.14307522504504927No Hit
GATAGATCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA309820.13265371745109278No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCAGTAGGTCCACTAT291760.12492107870224914No Hit
GATAAGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA280680.1201770234787061No Hit
GATAGGTCACATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA275570.11798910631333559No Hit
AATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA255220.10927597239644923No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACGCCAGTAGGTCCACTAT235840.10097815739353727No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCGC23163450.0144.915054
CGCATCT23205750.0144.857048
GGTCGCA23173550.0144.841235
GATAGGT23186100.0144.809451
TAGGTCG23179800.0144.800313
GCATCTT23206750.0144.768029
TCGCATC23226950.0144.733237
ATAGGTC23230350.0144.718112
GTCGCAT23215750.0144.65266
ACGTCTG22424100.0131.60722145
CGTCTGA2086300.0110.859314145
ACGACTG65850.0107.34451145
ATCGCAT49250.0100.5662846
ACGTCGA68350.090.26556145
ACGTCTA81050.089.62841145
ATCTCGT121250.086.63984145
AGATCGC59150.083.856284
AAGTCGC54350.081.655624
GATAAGT56600.078.664991
ATAAGTC58850.075.656922