FastQCFastQC Report
Tue 29 Sep 2020
HVC5JBGXG_n01_0920_CD4_CD_high.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC5JBGXG_n01_0920_CD4_CD_high.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14290225
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA1333393993.30811096396313No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCCA344050.24075898035195387No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCA285000.19943702775848526No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACAGCCAGTAGGTCCA260770.18248138150379017No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCAC211780.14819920610067372No Hit
ATCGATTCCTTGGTTCTTGTCGAAAGGACGAAACACCGCCAGTAGGTCCA200990.14064859020764192No Hit
ATCGATTCCTTGGTTCTTGTGGAAAAGACGAAACACCGCCAGTAGGTCCA195170.13657587616710024No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCAGTAGGTCCAC181800.1272198303385706No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTCCA179760.12579228108724672No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGAACGAAACACCGCCAGTAGGTCCA170170.11908140004793487No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCCCCAGTAGGTCCA168580.11796875136675594No Hit
ATCGATTCCTTGGTTCTTATGGAAAGGACGAAACACCGCCAGTAGGTCCA160670.11243349912265202No Hit
ATCGATTCCTTGGTTCTTGTAGAAAGGACGAAACACCGCCAGTAGGTCCA159950.11192965821042007No Hit
ATCGATTCCTTGGTTCTTGTGAAAAGGACGAAACACCGCCAGTAGGTCCA150930.1056176512266252No Hit
ATCGATTCCTTGATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA149850.10486188985827725No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACGCCAGTAGGTCCAC144010.10077518023684023No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCC14205550.0144.868733
TCGATTC14214700.0144.855592
ATTCCTT14225950.0144.840425
GATTCCT14216500.0144.827034
ATCGATT14219900.0144.795491
TTCCTTG14214750.0144.77146
CCTTGGT14195250.0144.736548
TCCTTGG14198550.0144.736077
CTTGGTT14199100.0144.617139
GCACACG13700200.0133.63132145
GCACACC139650.0130.5653145
CACACGT1084450.0114.793365145
CCTTAGT23100.087.253598
GAACACG33250.080.4573145
GCACACT39650.072.59021145
AATTCCT27800.069.647444
GACACGT21850.068.01946145
CTATTCC33150.065.844133
CAATTCC29450.065.74463
ATCAATT30600.063.2724761
Produced by FastQC (version 0.11.5)