FastQCFastQC Report
Wed 31 Oct 2018
HVC57BGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC57BGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16767846
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2858571.7047926131955171No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCTTTCCCTACACGA243070.14496197066695388TruSeq Adapter, Index 13 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC30750.056.10045
TACGGCG31100.055.3565376
TACACGG19550.048.89696525
TGATACG37600.046.531983
GATACGG37700.046.315324
ACACGGC21200.044.93082426
GATCTAC37250.044.26121521
CTCCAGT221550.041.9583724
CAGTCAC201600.041.11874427
GAACTCC250300.040.6968521
CCAGTCA210850.040.67490826
CTGAACT261050.040.12925719
TGAACTC264350.039.63150420
CGTCTGA267700.039.41193416
AACTCCA259650.039.20661522
CACCGAG42600.039.01846715
ACGTCTG272600.038.75461615
CGGCTCT24600.037.44201329
GAGATCT44450.037.1662219
CCACCGA44800.037.1021614
Produced by FastQC (version 0.11.5)