FastQCFastQC Report
Wed 31 Oct 2018
HVC57BGX7_n01_9_biotin_NAC4_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC57BGX7_n01_9_biotin_NAC4_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20954902
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC4092331.9529225190363573TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG390100.18616169142666475TruSeq Adapter, Index 9 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT595000.055.68148449
TATGCCG602950.055.42324446
ATGCCGT604950.055.12431347
CGTATGC610100.054.8598944
TGCCGTC608000.054.80749548
GTATGCC610750.054.76126545
CTCGTAT599000.053.7323442
CCGTCTT617650.053.667950
TCGTATG630350.053.06963343
CTGCTTG622600.053.04997657
TCTCGTA598900.052.9115641
ATCTCGT607350.051.2081140
TGCTTGA650350.050.9643158
GCTTGAA655950.050.66792759
GATCTCG619550.050.13243539
CTTCTGC670350.049.3912254
GTCACGA685000.048.7962829
CGATCAG684350.048.74947733
CACGATC685100.048.71933731
AGATCTC638150.048.69970338
Produced by FastQC (version 0.11.5)