FastQCFastQC Report
Wed 31 Oct 2018
HVC57BGX7_n01_8_biotin_HHO3_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC57BGX7_n01_8_biotin_HHO3_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35040060
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC853550.24359261941903068TruSeq Adapter, Index 8 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC159400.046.12720544
GTATGCC171350.044.27860645
TATGCCG175700.042.72417446
CTCGTAT183950.038.44902442
TCGTATG186950.037.4575343
GCCGTCT171350.037.29364449
ATGCCGT193200.037.18775647
TCTCGTA208750.033.79741341
TGCCGTC221400.031.5659348
CCGTCTT211700.031.14422850
ATCTCGT276950.026.6247240
AATCTCG314050.024.46016939
CTGCTTG249400.024.20518157
AAAAGGG314150.022.3378870
CGTCTTC301700.021.19233951
CTTCTGC303950.021.16211354
GCTTGAA315100.020.97978859
TGCTTGA308200.020.40477258
GAATCTC397600.019.58457638
TCTGCTT349850.018.12558256
Produced by FastQC (version 0.11.5)