FastQCFastQC Report
Tue 30 Oct 2018
HVC57BGX7_n01_7_biotin_TGA4_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC57BGX7_n01_7_biotin_TGA4_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21130441
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1272990.6024436498982677TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG184700.058.6044246
CGTATGC187150.057.6315344
ATGCCGT185900.056.43773747
GTATGCC194150.056.05835345
GCCGTCT179250.054.90014649
TGCCGTC193450.053.94562548
CTCGTAT188450.052.5912442
TCGTATG196100.051.5746843
TCTCGTA201300.048.4692541
CCGTCTT210000.047.4611350
CATCTCG217600.046.47982839
ATCTCGT223300.044.5562440
CAGATCA290850.042.12766634
CTGCTTG227750.041.5186557
AGTCACC307450.040.67020428
GTCACCA313950.039.8834629
CAGTCAC325550.038.4517627
GATCATC299250.038.4447836
CACCAGA328050.037.996131
ACCAGAT334500.037.35676632
Produced by FastQC (version 0.11.5)