FastQCFastQC Report
Tue 30 Oct 2018
HVC57BGX7_n01_6_biotin_NLP7_3_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC57BGX7_n01_6_biotin_NLP7_3_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16258342
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2178041.339644595986479TruSeq Adapter, Index 6 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG214820.1321290940982789TruSeq Adapter, Index 6 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG285550.058.3484946
CGTATGC288850.057.83956544
GCCGTCT288650.056.95769549
ATGCCGT291950.056.91322347
GTATGCC294250.056.67089545
TGCCGTC295650.056.04708548
CTCGTAT293750.056.0050742
TCGTATG302900.055.0411443
TCTCGTA305600.053.9709941
CCGTCTT309100.053.2461650
TATCTCG312450.052.7208839
CGCCAAT325450.051.6911833
ATCTCGT320300.051.47232440
ACGCCAA330150.050.96779632
CACGCCA332000.050.7382331
GCCAATA335950.049.9888334
GTCACGC344800.048.8281229
AGTCACG347150.048.7300328
TCACGCC345750.048.7243430
CTGCTTG335650.048.5234957
Produced by FastQC (version 0.11.5)