FastQCFastQC Report
Tue 30 Oct 2018
HVC57BGX7_n01_5_biotin_NLP7_1_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC57BGX7_n01_5_biotin_NLP7_1_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14301738
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2290971.601882232774786TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC528650.3696403891610936TruSeq Adapter, Index 5 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG186010.13006111564902112TruSeq Adapter, Index 5 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG398800.057.80219346
CGTATGC398250.057.54810344
GCCGTCT375200.057.5112149
ATGCCGT394700.057.31206547
CTCGTAT318000.057.04921742
GTATGCC407600.056.88910745
TGCCGTC394500.056.73790448
TCTCGTA320500.055.69793741
TCGTATG396150.055.4768543
CCGTCTT396900.055.19548450
ATCTCGT332600.053.09387240
GATCTCG340100.052.44818539
CTGCTTG391950.052.24971857
CAGTGAT465350.051.5198635
AGTGATC372250.051.01714336
GTGATCT354950.051.00626837
CACAGTG498200.050.93204533
ACACAGT508550.049.93853432
AAAAGGG376400.049.8200870
ACAGTGA496600.049.68819834
Produced by FastQC (version 0.11.5)