FastQCFastQC Report
Tue 30 Oct 2018
HVC57BGX7_n01_4_biotin_CRF4_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC57BGX7_n01_4_biotin_CRF4_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6777616
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1379652.035597767710652TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG137670.2031245204803577TruSeq Adapter, Index 4 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG196750.058.4656346
CGTATGC198300.058.0968844
GCCGTCT196300.057.9570149
GTATGCC199450.057.77944645
ATGCCGT198900.057.7272347
TGCCGTC201500.056.8781648
CTCGTAT190700.056.74197842
TCGTATG205000.055.99324843
CCGTCTT203300.055.9618550
TCTCGTA190100.055.92697541
ATCTCGT195650.053.69775440
ACTGACC215900.052.80148332
CTGCTTG212950.052.65312657
AATCTCG199250.052.5702539
CTGACCA220750.051.5760633
GACCAAT213750.051.5685335
GCTTGAA220450.051.2749159
TGCTTGA219800.051.12403558
CCAATCT207650.050.36309437
ACCAATC206250.050.31651336
Produced by FastQC (version 0.11.5)