FastQCFastQC Report
Tue 30 Oct 2018
HVC57BGX7_n01_3_biotin_TGA4_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC57BGX7_n01_3_biotin_TGA4_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9907216
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC2044922.064071278954653TruSeq Adapter, Index 3 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG156060.15752154793031664TruSeq Adapter, Index 3 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC282100.060.26753644
TATGCCG284250.059.93419646
GTATGCC287450.059.5838345
GCCGTCT271850.059.5783749
ATGCCGT283750.059.27512747
CTCGTAT271750.058.59609242
TGCCGTC287550.058.13885548
TCGTATG283700.057.99061643
TCTCGTA271750.057.26991741
CCGTCTT286100.057.23446350
CATCTCG279200.056.0183839
ATCTCGT281350.055.11733640
GCATCTC286200.054.92950438
TAGGCAT319750.054.55028535
GGCATCT292750.054.1213837
CTGCTTG290250.053.6547357
TTAGGCA336800.053.64468434
AGGCATC314350.052.68814536
GCTTGAA311400.052.24724259
TGCTTGA304050.052.2093558