Basic Statistics
Measure | Value |
---|---|
Filename | HVC57BGX7_n01_3_biotin_TGA4_R1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 9907216 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC | 204492 | 2.064071278954653 | TruSeq Adapter, Index 3 (100% over 50bp) |
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG | 15606 | 0.15752154793031664 | TruSeq Adapter, Index 3 (100% over 49bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 28210 | 0.0 | 60.267536 | 44 |
TATGCCG | 28425 | 0.0 | 59.934196 | 46 |
GTATGCC | 28745 | 0.0 | 59.58383 | 45 |
GCCGTCT | 27185 | 0.0 | 59.57837 | 49 |
ATGCCGT | 28375 | 0.0 | 59.275127 | 47 |
CTCGTAT | 27175 | 0.0 | 58.596092 | 42 |
TGCCGTC | 28755 | 0.0 | 58.138855 | 48 |
TCGTATG | 28370 | 0.0 | 57.990616 | 43 |
TCTCGTA | 27175 | 0.0 | 57.269917 | 41 |
CCGTCTT | 28610 | 0.0 | 57.234463 | 50 |
CATCTCG | 27920 | 0.0 | 56.01838 | 39 |
ATCTCGT | 28135 | 0.0 | 55.117336 | 40 |
GCATCTC | 28620 | 0.0 | 54.929504 | 38 |
TAGGCAT | 31975 | 0.0 | 54.550285 | 35 |
GGCATCT | 29275 | 0.0 | 54.12138 | 37 |
CTGCTTG | 29025 | 0.0 | 53.65473 | 57 |
TTAGGCA | 33680 | 0.0 | 53.644684 | 34 |
AGGCATC | 31435 | 0.0 | 52.688145 | 36 |
GCTTGAA | 31140 | 0.0 | 52.247242 | 59 |
TGCTTGA | 30405 | 0.0 | 52.20935 | 58 |