FastQCFastQC Report
Tue 30 Oct 2018
HVC57BGX7_n01_2_biotin_HHO3_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC57BGX7_n01_2_biotin_HHO3_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10316648
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1753321.6995054982975089TruSeq Adapter, Index 2 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG159290.15440092557194934TruSeq Adapter, Index 2 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT225300.056.8197849
TATGCCG242150.056.59463546
CGTATGC245600.055.913944
GTATGCC248350.055.61859545
ATGCCGT241750.055.53019347
TGCCGTC243950.054.6420848
CTCGTAT241550.054.5332942
CCGTCTT240400.054.0655150
TCGTATG245250.054.02449443
TCTCGTA246150.053.00287641
TATCTCG256950.052.0969739
ATCTCGT260150.050.79697440
CTGCTTG240250.050.5742157
CGATGTA304500.049.80463834
CCGATGT312000.048.67874533
GTATCTC278300.048.46593538
ACCGATG317300.047.97807732
AAAAGGG230050.047.93859570
TGCTTGA258400.047.8480258
GCTTGAA267800.047.77631859
Produced by FastQC (version 0.11.5)