FastQCFastQC Report
Tue 30 Oct 2018
HVC57BGX7_n01_24_input_HSF_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC57BGX7_n01_24_input_HSF_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21502914
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGC2280271.0604469701176316TruSeq Adapter, Index 7 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATG509440.23691672672829367TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC359950.051.5095144
TATGCCG360300.051.4786646
ATGCCGT359450.051.30819347
GTATGCC362850.051.22297345
TGCCGTC360100.050.9143148
GCCGTCT355350.050.77748549
TCTCGTA358200.048.31259541
CTCGTAT366500.047.8675742
CCGTCTT380350.047.7159650
ATCTCGT366750.046.96769340
CCACTCA410850.046.03315433
TCGTATG401650.045.84792743
AGTCACC428250.044.5443628
GCTTGAA418150.043.01754459
ACCACTC441450.042.97879832
TGCTTGA414750.042.86392258
AATCTCG405150.042.65471339
GTCACCA447550.042.5685829
CTGCTTG427750.041.53658357
CACTCAA464300.040.48198734
Produced by FastQC (version 0.11.5)