FastQCFastQC Report
Tue 30 Oct 2018
HVC57BGX7_n01_23_input_BZIP3_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC57BGX7_n01_23_input_BZIP3_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1783671
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGC32001317.94125710402871TruSeq Adapter, Index 7 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATG112270.629432221525158TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGCC83470.46796746709454823TruSeq Adapter, Index 7 (97% over 34bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG46790.2623241617988968Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATATCGTATGC33550.18809522608149148TruSeq Adapter, Index 7 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32610.18282519590215907No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT32310.18114327137684025Illumina Single End PCR Primer 1 (96% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCGCGTATGC21750.1219395280856167TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT384000.063.77948849
TGCCGTC387850.063.73290648
ATGCCGT389600.063.6352747
CCGTCTT385250.063.59979650
TATGCCG391050.063.5783146
GTATGCC391900.063.49399645
CGTATGC392100.063.39912844
CTCGTAT385000.063.27748542
TCTCGTA383950.063.20442241
TCGTATG391500.063.16553543
GCTTGAA383900.063.1032659
TGCTTGA380100.063.0619858
CTGCTTG378500.063.05116757
TATCTCG387750.062.74923739
CTTGAAA388800.062.74004460
ATCTCGT388950.062.6096340
TCTGCTT383400.062.49180656
TTCTGCT381300.062.26691455
CTTCTGC387100.062.0572354
TCTTCTG389050.061.91710753
Produced by FastQC (version 0.11.5)