FastQCFastQC Report
Tue 30 Oct 2018
HVC57BGX7_n01_22_input_HHO2_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC57BGX7_n01_22_input_HHO2_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14734481
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC171390.11631899352274437TruSeq Adapter, Index 23 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG34850.042.17546
GCCGTCT38000.038.03400449
GTATGCC38200.037.83525545
CGTATGC48900.029.55637644
ATGCCGT51900.028.31962847
TCTCGTA52600.025.28187841
CTCGTAT56100.024.8897242
TCGTATG60850.024.38458343
ATCTCGT60000.022.51388740
CCGTCTT66350.022.04656650
AGTCACC73350.019.86032128
TGCCGTC80250.018.5331648
CCAACTA72400.018.42542633
GTCACCA74050.018.30092629
GCTTGAA81500.018.12009459
TGCTTGA81500.017.73364658
ACGACGC15350.017.32649847
AATCTCG86850.015.39256239
CACCAAC90950.015.20693631
CAACTAA90350.015.07391534
Produced by FastQC (version 0.11.5)