FastQCFastQC Report
Tue 30 Oct 2018
HVC57BGX7_n01_21_input_NAC4_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC57BGX7_n01_21_input_NAC4_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35299876
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC68850.036.69863544
TATGCCG68500.036.32401346
ATGCCGT71900.034.84969347
GTATGCC73850.034.1190945
GCCGTCT73600.033.9020549
TGCCGTC73750.033.8331448
CCGTCTT113400.022.21937650
TCTCGTA111450.021.8233541
TCGTATG126150.021.05577543
CTCGTAT123800.020.8335642
ATCTCGT123100.019.24662840
AGTCACC164950.015.17809128
TGCTTGA175250.014.59729458
GCTTGAA187650.013.96850659
CTGCTTG195300.013.439157557
GTCACCA205950.012.36039529
AGATCTC197600.012.0967938
TCTGCTT233900.011.19139956
TGGGGGG39100.010.5664561
CCAAAAG244500.010.45338633
Produced by FastQC (version 0.11.5)