FastQCFastQC Report
Tue 30 Oct 2018
HVC57BGX7_n01_20_input_HHO3_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC57BGX7_n01_20_input_HHO3_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14380435
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG22000.025.133348
CGTATGC23200.024.13498946
GTATGCC24950.022.86294647
GCCGTCT26050.021.36015951
TGGGGGG23750.014.4472271
CCGTCTT42650.013.45674252
ATGCCGT43000.013.42859649
TCTCGTA41300.013.30346343
TCGTATG46450.012.95859345
CTCGTAT45450.012.55072644
ATCTCGT48550.011.53309442
TATCTCG48750.011.41399341
GTCACAT57250.010.520184529
CACATTC67750.09.04414331
TGCTTGA72300.08.18019360
CGTCTGA211750.08.16508970
CACACGT331250.08.11451870
ACATTCC76100.08.05161532
GCTTGAA72750.07.98528161
TGAACTC149700.07.90227870
Produced by FastQC (version 0.11.5)