FastQCFastQC Report
Tue 30 Oct 2018
HVC57BGX7_n01_1_biotin_ASR3_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC57BGX7_n01_1_biotin_ASR3_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5982027
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1557772.6040838665556008TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG111030.18560598272124149TruSeq Adapter, Index 1 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG228350.057.30333346
GCCGTCT227450.057.03771249
ATGCCGT230450.056.79633347
CGTATGC231400.056.72952344
GTATGCC231100.056.65173345
TGCCGTC230900.056.62501548
CTCGTAT221350.056.0794142
CCGTCTT232450.055.94585450
TCTCGTA216450.055.74827641
CTGCTTG232350.055.50255657
TCGTATG236550.055.09453643
ATCTCGT218650.053.93936540
CACGATC220100.053.78111636
TGCTTGA240850.053.63095558
ACGATCT219900.053.47762737
CGATCTC220850.053.4029438
TCACGAT232050.053.219935
GATCTCG222750.052.9312739
GCTTGAA246000.052.86472759
CTTCTGC244950.052.7904254