Basic Statistics
Measure | Value |
---|---|
Filename | HVC57BGX7_n01_1_biotin_ASR3_R1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 5982027 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC | 155777 | 2.6040838665556008 | TruSeq Adapter, Index 1 (100% over 50bp) |
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG | 11103 | 0.18560598272124149 | TruSeq Adapter, Index 1 (100% over 49bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 22835 | 0.0 | 57.303333 | 46 |
GCCGTCT | 22745 | 0.0 | 57.037712 | 49 |
ATGCCGT | 23045 | 0.0 | 56.796333 | 47 |
CGTATGC | 23140 | 0.0 | 56.729523 | 44 |
GTATGCC | 23110 | 0.0 | 56.651733 | 45 |
TGCCGTC | 23090 | 0.0 | 56.625015 | 48 |
CTCGTAT | 22135 | 0.0 | 56.07941 | 42 |
CCGTCTT | 23245 | 0.0 | 55.945854 | 50 |
TCTCGTA | 21645 | 0.0 | 55.748276 | 41 |
CTGCTTG | 23235 | 0.0 | 55.502556 | 57 |
TCGTATG | 23655 | 0.0 | 55.094536 | 43 |
ATCTCGT | 21865 | 0.0 | 53.939365 | 40 |
CACGATC | 22010 | 0.0 | 53.781116 | 36 |
TGCTTGA | 24085 | 0.0 | 53.630955 | 58 |
ACGATCT | 21990 | 0.0 | 53.477627 | 37 |
CGATCTC | 22085 | 0.0 | 53.40294 | 38 |
TCACGAT | 23205 | 0.0 | 53.2199 | 35 |
GATCTCG | 22275 | 0.0 | 52.93127 | 39 |
GCTTGAA | 24600 | 0.0 | 52.864727 | 59 |
CTTCTGC | 24495 | 0.0 | 52.79042 | 54 |