FastQCFastQC Report
Tue 30 Oct 2018
HVC57BGX7_n01_19_input_TGA4_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC57BGX7_n01_19_input_TGA4_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15970457
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGC1207330.7559771144933423TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGCGTATGC209380.1311045764062982TruSeq Adapter, Index 1 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG194000.057.61692446
ATGCCGT183300.057.35276447
GTATGCC200000.056.99096345
CGTATGC192600.056.89119344
TGCCGTC182200.056.52736748
GCCGTCT168900.055.9227849
CTCGTAT195100.052.6823242
CATCTCG220050.051.7985239
GCATCTC225450.051.17986738
TCGTATG193700.051.12991343
TCTCGTA201950.050.58329441
CCGTCTT197850.050.16309450
ATCTCGT217750.049.7098840
GTCACAT362900.047.00881629
TGAGCAT356250.045.85659435
CATGAGC373300.045.6476933
CATCGCG59600.045.5655339
CTGCGTG36000.044.8138857
TCACATG382000.044.80489330
ATGAGCA388150.043.58284434
Produced by FastQC (version 0.11.5)