FastQCFastQC Report
Tue 30 Oct 2018
HVC57BGX7_n01_18_input_NLP7_3_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC57BGX7_n01_18_input_NLP7_3_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15233691
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG26100.031.37562848
CGTATGC26750.030.35157646
GTATGCC29200.028.2843547
ATGCCGT29050.028.06899349
TGCCGTC31050.026.48633650
GCCGTCT40200.020.5446551
CTCGTAT44200.018.60641344
TCGTATG46600.018.2489345
CACACTG50450.017.21121231
TCTCGTA47850.016.89445743
ACTGATA58400.014.86668434
TATCTCG56650.014.76432641
ATCTCGT56250.014.74483942
CTGATAT62150.014.30594535
CCGTCTT60900.013.56149352
GTCACAC66250.013.47732729
ACACTGA67300.013.10981232
TCACACT90900.010.55432430
TGCTTGA82000.010.5414860
GCTTGAA84500.010.39526461
Produced by FastQC (version 0.11.5)