FastQCFastQC Report
Tue 30 Oct 2018
HVC57BGX7_n01_17_input_NLP7_1_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC57BGX7_n01_17_input_NLP7_1_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16827835
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG27950.030.42534646
CGTATGC28850.029.4763844
ATGCCGT30450.028.27215447
GTATGCC30400.027.97330545
TGCCGTC32900.025.84767348
CGGTAGC36550.024.2361533
ACGGTAG45600.019.96402432
GGTAGCA45800.019.339434
GCCGTCT44100.019.12447549
CACGGTA47950.018.69384431
TCGTATG51150.017.3096643
CTCGTAT49200.017.2844242
GTAGCAT51100.016.8513135
TCTCGTA51600.016.41267241
CATCTCG53350.015.67783839
GTCACGG58450.015.27694629
AGTCACG59600.014.92337328
ATCTCGT59250.014.47093340
TAGCATC58550.014.46630536
GCATCTC58950.014.12935438