FastQCFastQC Report
Tue 30 Oct 2018
HVC57BGX7_n01_16_input_CRF4_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC57BGX7_n01_16_input_CRF4_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15634612
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT190330.12173631171659392TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG33750.041.06160448
CGTATGC35600.039.61590246
GCCGTCT35000.039.49475551
GTATGCC35850.038.85155547
ATGCCGT36050.038.53869249
TGCCGTC36150.038.52889350
CCGTCTT49600.028.22205552
TCACGAG52350.027.69152330
TCTCGTA51550.026.67954343
TCGTATG56650.025.2660845
CTCGTAT56300.024.7394844
TATCTCG63050.022.09078441
GTGGATA72500.020.22813236
TGGATAT77950.019.08216337
AGTCACG76500.018.95005228
ATCTCGT74700.018.55198942
TGCTTGA83300.016.88889160
GCTTGAA83450.016.81659761
ACGAGTG88950.016.68910632
CGAGTGG91000.016.31288133
Produced by FastQC (version 0.11.5)