FastQCFastQC Report
Tue 30 Oct 2018
HVC57BGX7_n01_15_input_TGA4_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC57BGX7_n01_15_input_TGA4_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17842462
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG30900.037.48744648
CGTATGC30800.036.70017646
GTATGCC34000.034.06947347
ATGCCGT32550.033.65190549
CGTTTCG62250.031.89055633
TGCCGTC34600.030.2419850
GTCACGT70650.027.85376729
GCCGTCT34450.027.529351
GTTTCGG72400.027.07975634
CACGTTT79950.024.7873331
ACGTTTC81650.024.35646232
AGTCACG86850.022.81964328
TCTCGTA55850.021.24187543
CTCGTAT56350.020.74287244
TCGTATG57400.020.11955645
CCGTCTT53300.019.4347852
ATCTCGT67150.019.02230842
CGGAATC97100.018.74190938
TCACGTT126850.015.9273230
TCGGAAT128350.015.6520137
Produced by FastQC (version 0.11.5)