FastQCFastQC Report
Tue 30 Oct 2018
HVC57BGX7_n01_14_input_HHO3_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC57BGX7_n01_14_input_HHO3_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17978769
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT227820.12671612834004373TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC44550.042.96896746
TATGCCG44950.042.66444848
GTATGCC46550.041.4987247
GCCGTCT48150.039.465551
ATGCCGT62450.030.93290349
CACGTGA69750.028.06019431
CCGTCTT68300.027.97602752
TCGTATG70550.027.5845
TCACGTG71900.027.3198830
TCTCGTA66150.027.24551843
GTCACGT71350.027.13811129
CTCGTAT69300.026.15858344
CGTGAAA78150.024.99828133
CGATCTC69350.024.57565140
ATCTCGT73550.023.69541742
AGTCACG86900.022.60432428
ACGTGAA91800.021.54848532
GAAACGA89350.020.95716336
TGCCGTC95850.020.2270150
GATCTCG88850.019.69377941
Produced by FastQC (version 0.11.5)