FastQCFastQC Report
Tue 30 Oct 2018
HVC57BGX7_n01_13_input_ASR3_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC57BGX7_n01_13_input_ASR3_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17450213
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC191120.10952301842963177TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG37300.042.3143546
CGTATGC37850.041.9768644
ATGCCGT40450.039.19197547
GTATGCC41800.038.1775945
TGCCGTC42200.037.15207348
GCCGTCT53400.029.49094849
TCTCGTA59050.026.0767141
CTCGTAT60550.026.06640442
TCGTATG63800.025.28709643
ACGTAGA63950.024.63732732
GTCACGT65600.023.85902629
CGTAGAG73050.021.71161533
ATCTCGT69150.021.25602540
CCGTCTT74400.021.21386750
CACGTAG78650.020.03308731
AGTCACG80400.019.51089728
TCACGTA85950.018.41356330
AGATCTC90800.016.4580938
TGCTTGA97600.016.38631858
GAGATCT92550.016.29898837
Produced by FastQC (version 0.11.5)