FastQCFastQC Report
Tue 30 Oct 2018
HVC57BGX7_n01_12_biotin_HSF_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC57BGX7_n01_12_biotin_HSF_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10453125
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC112309910.744145889387145TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG1007390.963721375186846TruSeq Adapter, Index 12 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC537120.5138367713004484TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATGC183450.17549775784753363TruSeq Adapter, Index 12 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG167670.1604017937219731No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATG124500.11910313901345293TruSeq Adapter, Index 12 (97% over 49bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC122360.1170559043348281TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAGCTCGTATGC111930.1070780269058296TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATGC108440.10373931240657698TruSeq Adapter, Index 12 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1403650.060.643642
AAAAGGG1140450.060.61293470
GTATGCC1520850.060.43427345
TCTCGTA1398800.060.4038341
CGTATGC1519900.060.36872544
TATGCCG1519650.060.32050746
GCCGTCT1472850.060.3173849
CCGTCTT1484250.060.12289450
TCGTATG1514200.060.09647843
ATGCCGT1519400.059.91124347
CTGCTTG1447050.059.60534357
GCTTGAA1478000.059.57002359
TGCCGTC1519550.059.54837448
TGCTTGA1456700.059.53988658
TCTGCTT1465700.059.3315956
ATCTCGT1423250.059.30525240
CTTCTGC1470500.059.24024654
AATCTCG1428750.059.21308539
TTCTGCT1444000.059.1883855
GTAATCT1442250.058.92241737
Produced by FastQC (version 0.11.5)