FastQCFastQC Report
Tue 30 Oct 2018
HVC57BGX7_n01_11_biotin_BZIP3_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC57BGX7_n01_11_biotin_BZIP3_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11749728
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC2279361.9399257582813831TruSeq Adapter, Index 11 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG145310.12367094795726334TruSeq Adapter, Index 11 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG317300.059.33796746
GTATGCC318650.059.0206845
CGTATGC318850.059.0058644
GCCGTCT314850.058.89937649
ATGCCGT321250.058.46674747
TGCCGTC321800.058.2471948
CTCGTAT319150.057.45884742
TCGTATG326450.057.39607243
TCTCGTA319450.056.29841641
CCGTCTT329500.056.19518750
CATCTCG320050.055.3953639
GCTACAT330050.055.25514235
CGGCTAC342150.055.2354633
ACGGCTA343150.055.20806532
CACGGCT343500.055.1536931
CTACATC327600.054.5199936
CTGCTTG337750.054.29387757
ATCTCGT327850.054.15215340
AGTCACG351700.054.05898728
GTCACGG351850.054.016529
Produced by FastQC (version 0.11.5)