FastQCFastQC Report
Tue 30 Oct 2018
HVC57BGX7_n01_10_biotin_HHO2_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVC57BGX7_n01_10_biotin_HHO2_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17125421
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC6049383.5323978312708344TruSeq Adapter, Index 10 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG574280.3353377414780051TruSeq Adapter, Index 10 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC531830.31055002968978107TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATTC179960.10508354801905308TruSeq Adapter, Index 10 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG848450.060.7309646
GCCGTCT755900.060.33796349
CGTATGC848800.060.29792444
GTATGCC869250.060.18760345
ATGCCGT819250.060.17879547
TGCCGTC817250.059.79080248
CTCGTAT820050.059.5226442
TCGTATG815350.059.12322243
TCTCGTA824250.059.10062841
CCGTCTT800050.058.55217450
TATCTCG887450.058.50525739
AAAAGGG793550.057.94503870
CACTAGC1110050.057.9139231
ATCTCGT872250.057.90301540
GCTTATC1061800.057.3525936
ACTAGCT1120400.057.2204932
GTCACTA1129050.057.1099729
TCACTAG1129550.057.0220230
TAGCTTA1121450.056.92052534
CTGCTTG747600.056.82267857
Produced by FastQC (version 0.11.5)