FastQCFastQC Report
Wed 22 Nov 2017
HV7VKAFXX_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV7VKAFXX_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17413248
Sequences flagged as poor quality0
Sequence length50
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTCA2663300.043.87126514
CCCCGAG1730900.043.8520368
TCCCCGA1417550.043.817857
GGTCTCC1607350.043.8060114
TCCACGA6407200.043.7857327
ACGAGGT7716050.043.75708810
GTCTCGA2126600.043.75378815
CACGAGG7175900.043.7208989
CCACGAG6913800.043.7160158
GGGCCTC229800.043.6215175
AGGTCTC11762750.043.61663413
CCCGAGG1845950.043.5860449
AAGTCCA946650.043.56984
GTCTCAG2531650.043.5281515
TGGGTCC479150.043.5236173
GTCCACG6477250.043.521786
GTCTCCT1384600.043.51987515
GCCCCGA524000.043.5188877
CAATGGG269350.043.5121151
CTCGGGG680350.043.44851718
Produced by FastQC (version 0.11.2)