FastQCFastQC Report
Thu 8 Aug 2019
HV7FHBGX7_n02_ba182.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV7FHBGX7_n02_ba182.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463327317
Sequences flagged as poor quality0
Sequence length99
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39366590.8496496657027456No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTCAGGCAGCGAGAG7792420.16818391046863312No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA5740330.12389362313381577No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAACAAGCGAGATAGGAA4699590.10143131707470639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT14961500.070.247781
AGCAGTG16829200.062.373712
GGTATCA17918300.058.30668
GTGGTAT18015600.058.1414456
GTATCAA18681200.055.8988279
AGTGGTA18972100.055.3601155
TGGTATC19368100.053.968947
GCAGTGG19647550.053.4181443
CAGTGGT20377100.051.5350234
ATCAACG17453850.032.08202710-11
CAACGCA18171800.030.82252512-13
ACGCAGA18465550.030.39268914-15
AGTACAT18701600.029.93838320-21
TCAACGC17651200.029.51622612-13
TATCAAC17916150.029.2123110-11
CGCAGAG18109650.028.96740316-17
AGAGTAC19631800.028.68327918-19
TACATGG19527800.028.49415622-23
GTACATG18527400.028.03806522-23
AACGCAG18741200.027.93659414-15
Produced by FastQC (version 0.11.5)