FastQCFastQC Report
Thu 8 Aug 2019
HV7FHBGX7_n01_ba182.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV7FHBGX7_n01_ba182.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463327317
Sequences flagged as poor quality0
Sequence length27
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATG1724050.018.8435631
ACGAGGA1769150.018.5448741
TGACTAG1833900.018.501811
TGCCAAA1744400.018.4274371
GACCTGG1631350.018.2473471
GATGCTA1866550.018.1984331
TGAGCCG1263200.018.0482331
GGGACCT1297250.018.0182231
CGTGAGC853950.017.862451
CGAGGAA799600.017.8051622
CGGACTG1166100.017.79191
CCTACCA1763650.017.7409131
GCTGCAG1596900.017.7267071
CGATTGA1681450.017.685541
CATGGCG1626350.017.655011
AAGGCAG1169850.017.632511
CAAGGCC1912450.017.6276471
AGACTAG1058500.017.540478
TATGCCC1704600.017.5014271
ACTAGTA1432850.017.4922359
Produced by FastQC (version 0.11.5)