FastQCFastQC Report
Thu 8 Aug 2019
HV7CMBGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV7CMBGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50226832
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTAG1452300.0143.88241
TCGATTC1156950.0143.681842
ACTGTAT1136750.0143.676713
CGATCAT1218650.0143.446151
GTATCTC1151650.0143.312356
TATCTCT1186700.0143.056477
ATTCCTT1161050.0143.050495
TCGTTAC774300.0143.02935
GATTCCT1164750.0142.678474
AGTCTTG1487500.0142.647748
AGAGTCT1479950.0142.636236
TGATCGT1237500.0142.573687
ACAATGG1443500.0142.565033
AATGGTC1455750.0142.534845
ATCGCGC1048100.0142.512622
ACGATCT1158200.0142.502015
AACAATG1453800.0142.443632
CATGATC1239050.0142.433325
GCATCTT1452700.0142.331339
GATCATG1231900.0142.302812
Produced by FastQC (version 0.11.5)